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KMID : 0380020040190020164
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 2 p.164 ~ p.167
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The Effects of CD-product Specificity upon the Enzyme (CGTase) Reaction Condition
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Choi Hee-Wook
Hong Soon-Gang
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Abstract
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Cyclodextrin glucanotransferase (EC 2.4.1.19, abbreviated as CGTase) is one of the most applied industrial enzymes that produces cyclodextrins from starch and related -1,4-glucans by intramolecular transglycosylation reaction upon Ca2＋/ dependent manner. The reaction of CLEC, -CGTases from Bacillus macerans with the soluble starch as a substrate reveals that the surfactants (SDS, N-octyl--D-glucoside) significantly affect not only the overall products of CDs but also their selectivity. The surfactants (SDS, Lubrol PX) trigger the increase of -CD production, but Triton x-100 and Tween 80 suppress -CD specificity. Organic solvents (dimethyl sulfoxide, formamide, 2-methyl-2,4-pentandiol, and ethylene glycol) also cause changes of total product and product selectivity.
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KEYWORD
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CGTase, cyclodextrin, CLEC, surfactant, organic solvent
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